Dynamics of seed dormancy and germination at high temperature stress is affected by priming and phytohormones in rapeseed (Brassica napus L.).

High temperature stress (HTS) imposes secondary dormancy (SD) also known as thermo-dormancy in many seeds. Priming by soil moisture however, may improve germination though under HTS it may compromise seed longevity. Knowledge of how HTS and priming affect dormancy status/viability loss of a particular crop seed species is essential in agriculture. Accordingly, control non-primed and hydro-primed seeds from Dk-xpower and Traper rapeseed cultivars with low and high potential for SD induction, respectively, were compared for germination behavior, response to GA and some phytohormone effectors under HTS. HTS reduced germination in non-primed Dk-xpower and Traper seeds mainly through the induction of thermo-inhibition/death and thermo-dormancy, respectively. Under HTS, GA3 application reduced thermo-dormancy in favor of thermo-inhibition only in Traper but the GA inhibitor paclobutrazol intensified thermo-dormancy in both cultivars. The ABA inhibitor, fluridone also reduced thermo-dormancy in favor of thermo-inhibition only in Traper. Thus, under HTS, GA biosynthesis is determinant in seed thermo-dormancy/thermo-inhibition dynamics. Hydropriming improved germination under HTS through reduced thermo-inhibition/death (Dk-xpower) and thermo-dormancy (Traper). Here, GA3 application increased death and compromised germination mainly in Dk-xpower. Paclubutrazol application however, increased thermo-dormancy by compromising thermo-inhibition/death in Traper. Overall, hydro-priming weakened seed phytohormonal germination responses. Controlled deterioration resulted in decreased longevity of hydro-primed seeds but induced SD in non-primed Traper seeds. Thus, down-regulation of GA biosynthesis may control differential induction of SD in rapeseed seeds under HTS while hydro-priming stimulates seed germination possibly through overcoming limitations in GA biosynthesis. The agricultural importance of these findings at the ecosystem scale is discussed.

Characterization of and genetic variation for tomato seed thermo-inhibition and thermo-dormancy.

BACKGROUND: Exposing imbibed seeds to high temperatures may lead to either thermo-inhibition of germination or thermo-dormancy responses. In thermo-inhibition, seed germination is inhibited but quickly resumed when temperatures are lowered. Upon prolonged exposure to elevated temperatures, thermo-dormancy may be induced and seeds are not able to germinate even at optimal temperatures. In order to explore underlying physiological and molecular aspects of thermo-induced secondary dormancy, we have investigated the physiological responses of tomato seeds to elevated temperatures and the molecular mechanisms that could explain the performance of tomato seeds at elevated temperature. RESULTS: In order to investigate how tomato seeds respond to high temperature we used two distinct tomato accessions: Solanum lycopersicum (cv. Moneymaker) (MM) and Solanum pimpinellifolium accession CGN14498 (PI). MM seeds did not germinate under high temperature conditions while seeds of PI reached a maximum germination of 80%. Despite the high germination percentage of PI, germinated seeds did not produce healthy seedling at 37 °C. By using a candidate gene approach we have tested if similar molecular pathways (abscisic acid (ABA) and gibberellic acid (GA)) present in lettuce and Arabidopsis, are regulating thermo-inhibition and thermo-dormancy responses in tomato. We showed that the ABA biosynthesis pathway genes NCED1 and NCED9 were upregulated whereas two of the GA-biosynthesis regulators (GA3ox1 and GA20ox1) were downregulated in tomato thermo-dormant seeds at elevated temperature. To identify novel regulators of tomato seed performance under high temperature, we screened a Recombinant Inbred Line (RIL) population derived from a cross between the two tomato accessions MM and PI for thermo-inhibition and dormancy induction. Several QTLs were detected, particularly for thermo-dormancy, which may be caused by new regulators of thermo-inhibition and thermo-dormancy in tomato. CONCLUSIONS: None of the genes studied in this research were co-locating with the detected QTLs. The new QTLs discovered in this study will therefore be useful to further elucidate the molecular mechanisms underlying the responses of tomato seeds to high temperature and eventually lead to identification of the causal genes regulating these responses.